Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Tulipa gesneriana |
Protein Variants | Comment | Organism |
---|---|---|
D327N | 0.1% of wild-type activity | Tulipa gesneriana |
G152A | 0.1% of wild-type activity | Tulipa gesneriana |
G153A | 0.01% of wild-type activity | Tulipa gesneriana |
G154A | 88% of wild-type activity | Tulipa gesneriana |
H359A | 0.001% of wild-type activity | Tulipa gesneriana |
H362A | 6% of wild-type activity | Tulipa gesneriana |
S235A | 0.01% of wild-type activity | Tulipa gesneriana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaF | 0.2 mM, 80% inhibition | Tulipa gesneriana | |
phenylmethylsulfonyl fluoride | 2 mM, 91% inhibition | Tulipa gesneriana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18 | - |
6-tuliposide A | pH 6.5, 22°C | Tulipa gesneriana | |
71 | - |
6-tuliposide B | pH 6.5, 22°C | Tulipa gesneriana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plastid | - |
Tulipa gesneriana | 9536 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
2 * 39000, SDS-PAGE | Tulipa gesneriana |
85400 | - |
gel filtration | Tulipa gesneriana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tulipa gesneriana | I4DST8 | isoform TCEA1 | - |
Purification (Comment) | Organism |
---|---|
- |
Tulipa gesneriana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | gene TCEA1 is transcribed in all tissues except for bulbs | Tulipa gesneriana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2260 | - |
pH 6.5, 22°C | Tulipa gesneriana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-tuliposide A | - |
Tulipa gesneriana | tulipalin A + D-glucose | enzyme accepts 6-tuliposide A to catalyze only the intramolecular transesterification but not the hydrolysis | ? | |
6-tuliposide B | 8.7% of the activity with 6-tuliposide A | Tulipa gesneriana | tulipalin B + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 39000, SDS-PAGE | Tulipa gesneriana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 45 | - |
Tulipa gesneriana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
stable up to, for 30 min | Tulipa gesneriana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
510 | - |
6-tuliposide B | pH 6.5, 22°C | Tulipa gesneriana | |
2800 | - |
6-tuliposide A | pH 6.5, 22°C | Tulipa gesneriana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Tulipa gesneriana |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | when maintained at room temperature | Tulipa gesneriana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.2 | - |
6-tuliposide A | pH 6.5, 22°C | Tulipa gesneriana | |
160 | - |
6-tuliposide A | pH 6.5, 22°C | Tulipa gesneriana |